PREVALENCE AND ANTIBIOGRAM OF SALMONELLA SPECIES IN CATTLE CARCASSES SLAUGHTER FROM ZANGO ABATTOIR ZARIA, NIGERIA

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PREVALENCE AND ANTIBIOGRAM OF SALMONELLA SPECIES IN CATTLE CARCASSES SLAUGHTER FROM ZANGO ABATTOIR ZARIA, NIGERIA

 

Abstract

 

Cross sectional study was conducted in Zango abattoir Zaria to determine the prevalence of Salmonella species in carcasses. A total of 200 pooled swab samples were collected from neck, brisket, flank and rump of cattle carcasses from February to March 2015. Ninety eight (49%) out of the two hundred (200) samples showed characteristics of Salmonella on Salmonellla Shigella Agar. Conventional biochemical tests were conducted on the 98 isolates, where 48 (48.9%) showed characteristics of Salmonella species. The 48 isolates were further subjected to Microbact 12E GNB identification system. The results showed that 19 (39.9%) were Salmonella arizonae, 3 (6.25%) were Salmonella Cholerasuis, and 2 (4.16%) were unclassified species. Other organisms detected included Serrattia liquefaciens 5 (10.4%), Escherichia coli 3(6.25%), Klebsiella pneumoniae 4 (8.33%), Edwardsiella tarda 2(4.16%), Citrobacter diversus 2(4.16%) and Hafnia alvei, Escherichia vulneris, Enterobacter gergoviae, Acinetobacter baumanni, Acinetobacter iwoffii, Enterobacter agglomerans, Klebsiella rhinoscleromatis and Klebsiella ozaenae 1 (2.08%) each. Twenty five (25) out of the 48 isolates did not meet the cut- off point of 75% for species identification. Agglutination test using Rapid Latex Agglutination was also performed, where 10 (55.55%) out of the 18 isolates agglutinated while 8 (44.44%) did not. Antibiotic susceptibility of these isolates using disk diffusion method revealed 100% susceptibility to gentamicin and varying percentages of susceptibility to the rest of the antibiotics. These were Ciprofloxacin (90%), Amikacin (70%), Chloramphenicol (60%), Nitrofurantoin (40%), Trimethoprim (40%), Nalidixic acid (30ug), Kenamycin (40%), Tetracycline (30%), Streptomycin (20%), Imipenem (20%) and Aoxicillin + Clavulanic acid (30%). All the isolates showed multiple drug resistance with the least showing resistance to 2 antibiotics and the highest to 7 antibiotics. In conclussion, prevalence of 5% of Salmonella species in cattle carcasses was recorded in this study with the isolates showing 100% suseptibilty to Gentamicin and 90% to Ciprofloxacin. This study demonstrated heavy contamination of carcasses with broad spectrum microbes that are of zoonotic importance and the use of Gentamicin and Ciprofloxacin should be with caution for their high potential antibiotic resistance to most of the zoonotic microbes.

 

PREVALENCE AND ANTIBIOGRAM OF SALMONELLA SPECIES IN CATTLE CARCASSES SLAUGHTER FROM ZANGO ABATTOIR ZARIA, NIGERIA

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