- : Ms Word Format
- : Pages
- : ₦3000
- : 1-5 Chapters
- Click to DOWNLOAD Materials
INVESTIGATING MOLECULAR SIGNATURES AND ANTIBIOTIC RESISTANCE OF NON-TYPHOIDAL SALMONELLA ISOLATES FROM FRESH PRODUCE
Abstract:
The contamination of fruits and vegetables by harmful microorganisms, also known as microbial contamination, has emerged as a critical public health concern due to its association with various foodborne disease outbreaks. In recent years, there has been an alarming rise in the global incidence of non-typhoidal Salmonella (NTS) contamination in fresh produce. This study aimed to investigate the prevalence and characteristics of Salmonella species in 63 fresh produce samples obtained from roadside vendors in Lagos and Ogun State.
Out of the 63 samples analyzed, 12 were found to be positive for Salmonella species growth on Xylose Lysine Deoxycholate (XLD) and Hektoen Enteric (HEA) agar plates. The identification of these 12 isolates as Salmonella species was further confirmed through rigorous microbiological tests, including Gram staining, catalase testing, and oxidase testing. Molecular typing was employed, utilizing Salmonella spp-specific polymerase chain reaction (PCR) techniques.
The antimicrobial susceptibility of the isolates was assessed using the Kirk-Bauer disk diffusion method. Alarmingly, all 12 isolates displayed antimicrobial resistance (AMR) profiles, demonstrating resistance to more than three antimicrobial agents. Consequently, these isolates were classified as multidrug-resistant (MDR) strains.
The presence of MDR Salmonella spp. in the fresh produce samples obtained from roadside vendors underscores a significant risk to human health. It emphasizes the urgent need for comprehensive precautions and measures to safeguard the entire supply chain, from farm to fork. Regulation of antibiotic use in farm animals is essential, and implementing antibiotic stewardship programs could play a pivotal role in mitigating the spread of antibiotic-resistant non-typhoidal Salmonella strains.
This study highlights the pressing importance of addressing microbial contamination in fresh fruits and vegetables to ensure food safety and protect public health.
INVESTIGATING MOLECULAR SIGNATURES AND ANTIBIOTIC RESISTANCE OF NON-TYPHOIDAL SALMONELLA ISOLATES FROM FRESH PRODUCE. GET MORE BIO TECHNOLOGY PROJECT TOPICS AND MATERIALS
